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Aluminium Oxide Chromatography

Aluminium Oxide Chromatography

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Aluminum Oxide is one of the strong adsorbent media which is highly preferred in column chromatography techniques. Active aluminum oxide is a typical amphoteric in nature (acting either as a base or an acid as well as being configured as neutral ) provides the best results for a chromatographer to separate a multitude of compounds over and above silica gels. Alumina can act as a weak ion exchanger demonstrating anionic or cationic properties, while additionally acting as an adsorbent. Alumina, due to its unique biological characteristics, is a special adsorbent for use in separation sciences. These active media are available in three different grades as per their application.

  • Acidic
  • Basic
  • Neutral

This active media is a white to off white fine particles, which are highly porous in nature. Available grades are very effectively sensitive to aromatic compounds. Mostly Alumina acidic works and provides useful separation with weak acids where these can be improved by using basic solvents.

Acidic & Basic alumina Oxide is used to do the separation of sensitive material.

Grades Alumina- Basic Alumina-Acidic Alumina-Neutral
pH (5 % aqueous Solution) 9.5 + 0.5 7.0 + 0.5 4.5 + 0.5
Specific Surface [m2/gm] 150 + 10% 150 + 10% 150 + 10%
Brockman Activity I -II I -II I -II
Mean Pore Diameter [A] 60 60 60
Pore Volume [ml/gm] ~ 0.25 ~ 0.25 ~ 0.25
CI [mval/g] Nil 0.03 0.14
Fe2O3 [%] 0.01 -0.03 0.01 -0.03 0.01-0.03
Na2O [%] 0.25 -04 0.29 -04 0.25 -04
Bulk Density [g/l] ~ 920 ~ 920 ~ 920
  • Isolation & purification of antibiotics
  • Dehydrations of organic solvents
  • Enzyme purification
  • Isolation of glycosides
  • Removal of peroxides from organic solvents
  • Purification of organic solvents.
  • Purification of Natural products & herbals like reserpine, Capsaicin and Oleo Resin.
  • Purification of Dyes, dye intermediates, alkaloids steroids, liquids, amino acids, Hormones, liphatic & aromatic hydrocarbons and vitamins.